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Image Search Results
Journal: Journal of Biological Chemistry
Article Title: Late SV40 Factor (LSF) Enhances Angiogenesis by Transcriptionally Up-regulating Matrix Metalloproteinase-9 (MMP-9)
doi: 10.1074/jbc.m111.298976
Figure Lengend Snippet: FIGURE 3. LSF induces MMP-9. A, human angiogenesis array was screened using conditioned media (CM) from the indicated cells. Representative expression levels of Angiogenin, PlGF, and IL-8 are shown. MMP-9 mRNA expression was detected in parental HepG3 cells and its LSF-overexpressing clones (B) and in parental QGY-7703 cells and its LSF dominant negative- expressingclones(C)byreal-timePCR.DatarepresentmeanS.E.*,p0.05. D, analysis of Pro- and active MMP-9 by Western blot analysis using condi- tioned media from the indicated cells. E, functionally active MMP-9 level was determined in the indicated cells by gelatin zymography.
Article Snippet: Human Angiogenesis Array—The expression levels of 55 angiogenesis associated proteins were analyzed using conditioned media from cells cultured in serum-free condition with the
Techniques: Expressing, Clone Assay, Dominant Negative Mutation, Western Blot, Zymography